![]() ![]() Viscous SC IFC also shows satisfactory ability to distinguish different types of cancer cells and different subtypes of human breast cancer cells. MC, and demonstrated that viscous non-conductive PEG solution achieved an improved sensitivity (7.92×) and signal-to-noise ratio (1.42×) in impedance measurement, with the accuracy maintained and free of clogging. Through modeling analysis and experiments, we confirmed the accuracy (error < 1.60% ± 4.71%) of SC w.r.t. By placing MC and SC in series in the same microfluidic chip, we established an evaluation platform to prove the hypothesis. Herein, we hypothesize that the viscosity of the non-conductive liquid is the key to the performance of SC, and propose to employ non-conductive viscous sheath flow in SC to unlock the tradeoff between sensitivity and throughput, while ensuring measurement accuracy. Current sheath constriction (SC) solutions lack systematic evaluation of the performance and proper guidelines for the sheath fluid. As a label-free and high-throughput single cell analysis platform, impedance flow cytometry (IFC) suffers from clogging caused by a narrow microchannel as mechanical constriction (MC). ![]()
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What would be a better date than today to share the news, right? Long story short: This tool is back for now! Yay! :D One I will be using as long as it works for now. I thought about new possible methods and a few came to my mind. I do get messages daily across Twitch, Twitter, Discord and even Steam asking if I could please revive this tool, showing the huge demand I've never expected. ![]() Over the course of the past two weeks, I received *loads* of messages from you all. On April 3rd, Twitch did shutdown the TMI chatters endpoint which was used to fetch chatterlists across all Twitch and in turn broke this tool.Īt this point I wasn't sure if I'd go ahead and try different ways to track bots again because this will become a bit more tricky now. This tool has been among one of the most requested and used tools I've built, with an average request count of ~5 per second (just for the bots-online list alone). 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